From theory to practice: understanding the challenges in the implementation of electrogenerated chemiluminescence for analytical applications

Author:

Giagu Gabriele,Fracassa Alessandro,Fiorani Andrea,Villani Elena,Paolucci Francesco,Valenti GiovanniORCID,Zanut Alessandra

Abstract

AbstractElectrogenerated chemiluminescence (ECL) stands out as a remarkable phenomenon of light emission at electrodes initiated by electrogenerated species in solution. Characterized by its exceptional sensitivity and minimal background optical signals, ECL finds applications across diverse domains, including biosensing, imaging, and various analytical applications. This review aims to serve as a comprehensive guide to the utilization of ECL in analytical applications. Beginning with a brief exposition on the theory at the basis of ECL generation, we elucidate the diverse systems employed to initiate ECL. Furthermore, we delineate the principal systems utilized for ECL generation in analytical contexts, elucidating both advantages and challenges inherent to their use. Additionally, we provide an overview of different electrode materials and novel ECL-based protocols tailored for analytical purposes, with a specific emphasis on biosensing applications. Graphical abstract

Funder

HORIZON EUROPE Framework Programme

MIUR

Alma Mater Studiorum - Università di Bologna

Publisher

Springer Science and Business Media LLC

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