Abstract
Benign prostatic hyperplasia (BPH), commonly seen in older men, can cause symptoms of discomfort, and may even need surgical intervention. Studies have shown the potential link between gut microbes and BPH, but the molecular association is not fully understood. Methods: Four-week-old male Sprague–Dawley rats (n = 16) were randomly allocated to normal control diet (ND, 10% fat) and high-fat diet-induced BPH (HFD, 45% fat) groups. Metagenomic analysis was used to examine the abundance and discrepancies in gut microbiota within the two groups after 24 weeks of feeding. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis was conducted to assess the biological functions of the differentially expressed genes. Results: Rats with HFD-induced obesity exhibited morphological abnormalities in their prostate tissues. Metagenomic analysis of the gut revealed that Firmicutes were the dominant phyla in the HFD group, whereas the ND group had a higher abundance of Spirochaetes. At the genus level, Ruminococcus spp exhibited greater abundance in the HFD group, whereas Treponema spp were more abundant in the ND group. KEGG analysis demonstrated that the differentially expressed genes were mainly enriched in the NOD-like receptor (NLR) signaling, PI3K-Akt signaling, estrogen-signaling, signalings associated with GABAergic synapses, pantothenate and CoA biosynthesis. Conclusion: The findings of our study indicated that there was a notable variation in the microbiota abundance within the intestinal tract of obese rats suffering from prostate hyperplasia. It is plausible that these differentially abundant bacteria played a role in the development of pathological alterations in the prostate through the facilitation of inflammatory responses; however, additional research is required to validate the findings.
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Data Availability
The metagenomic sequences were accessible at the National Center for Biotechnology Information SRA database with accession number PRJNA876316. (https://www.ncbi.nlm.nih.gov/bioproject?term=PRJNA876316&cmd=DetailsSearch).
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Funding
This study was supported by the National Natural Science Foundation of China (NSFC No. 82073304 to J.Z. and 82172684 to D.H.), and the National Key Research and Development Plan (2019YFC0121501 to D.H.).
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DH and JZ conceived and designed the study, and procured resources. LG performed the animal model and wrote the initial manuscript. AA performed the bioinformatics analysis. YC and YM were responsible for specimen collection. JLZ carried out the statistical analyses. XL was involved in the conception of the study and edited the manuscript. All authors read and approved the final manuscript. We thank Editage for English language editing.
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10528_2023_10634_MOESM1_ESM.tif
Supplementary file1 (TIF 5900 kb) The nonmetric multidimensional scaling (NMDS) analysis of the abundance of intestinal microbiota between the two groups
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Supplementary file2 (TIF 5791 kb) Venn diagram of the relationship between the significant abundance microbiota and functionally enriched pathways
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Gu, L., Alemasi, A., Chen, Y. et al. Gut Microbiota Disorders in Obesity-Associated Benign Prostatic Hyperplasia in Rats. Biochem Genet (2024). https://doi.org/10.1007/s10528-023-10634-z
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DOI: https://doi.org/10.1007/s10528-023-10634-z