Abstract
AbstractOur previous surveillance revealed that t203-like G9 (tentatively designated subtype G9-VI) rotaviruses re-emerged in 2010 in Beijing and rapidly prevailed over the G9-III subtype (the most common G9 subtype globally) and previously predominant G genotypes over the following two years. G9-VI belongs to the VP7 evolutionary lineage VI, which includes unusual and sporadic human rotaviruses from China (t203) and Japan. To obtain insight into the epidemiology, evolution, and transmission advantages of G9-VI rotavirus, we performed follow-up surveillance (2014-2017) and whole-genome analysis of 12 representative G9 strains. The results showed that the G9 genotype was predominant (77.4%), with a marked increase in prevalence (previously 43.5%). Within the G9 genotype, subtype G9-VI accounted for the majority (98.3%) of cases. The most prevalent P-genotype was P[8] (93.7%), within which subtype P[8]b was rare (0.7%). Phylogenetically, the G9-VI subtype strains in this study clustered closely with contemporary emerging human rotaviruses from many other countries in VP7 lineage VI, indicating that this subtype is capable of spreading globally. These currently emerging G9-VI rotaviruses formed a distinct monophyletic subcluster when compared to early G9-VI rotaviruses. Furthermore, four specific amino acid substitutions and synonymous codon substitutions were observed in the VP7 genes between the current G9-VI and globally common G9-III rotaviruses. The remaining nine genes of all of the analyzed representative G9 strains, whether G9-VI or G9-III, combined with the P[8]a, P[8]b, or P[6] genotype and exhibited the same Wa-like backbone constellation.
Funder
Beijing Municipal Science and Technology Commission
Research Foundation of Capital Institute of Pediatrics
Publisher
Springer Science and Business Media LLC
Subject
Virology,General Medicine
Reference36 articles.
1. Estes MK, Greenberg HB (2013) Rotaviruses. In: Knipe DM, Howley PM (eds) Fields virology. Wolters Kluwer Health/Lippincott, Williams and Wilkins, Philadelphia, pp 1347–1401
2. Troeger C, Khalil IA, Rao PC, Cao S, Blacker BF, Ahmed T et al (2018) Rotavirus vaccination and the global burden of rotavirus diarrhea among children younger than 5 years. JAMA Pediatr 172:958–965. https://doi.org/10.1001/jamapediatrics.2018.1960
3. Tate JE, Burton AH, Boschi-Pinto C, Parashar UD (2016) Global, regional, and national estimates of rotavirus mortality in children < 5 years of age, 2000–2013. Clin Infect Dis 62(Suppl2):S96–S105. https://doi.org/10.1093/cid/civ1013
4. ROTAVIRUS CLASSIFICATION WORKING GROUP: RCWG. https://rega.kuleuven.be/cev/viralmetagenomics/virus-classification/rcwg. Accessed 2 July 2021
5. Dóró R, László B, Martella V, Leshem E, Gentsch J, Parashar U et al (2014) Review of global rotavirus strain prevalence data from six years post vaccine licensure surveillance: is there evidence of strain selection from vaccine pressure? Infect Genet Evol 28:446–461. https://doi.org/10.1016/j.meegid.2014.08.017