Abstract
Porcine epidemic diarrhea virus (PEDV), porcine deltacoronavirus (PDCoV), and swine acute diarrhea syndrome coronavirus (SADS-CoV) cause intestinal diseases with similar manifestations in suckling piglets. In this study, we developed a multiplex real-time PCR for differential diagnosis of PEDV, PDCoV, and SADS-CoV. The assay demonstrated high specificity with a detection limit of 5 copies/µl for each virus. The assay specifically detected PEDV, PDCoV, and SADS-CoV and excluded all other swine pathogens circulating in pigs. Furthermore, the assay exhibited satisfactory performance in analyzing clinical samples. The data indicate that the newly developed multiplex real-time PCR method can be applied for differential diagnosis of porcine enteric coronaviruses.
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Data availability
The datasets used or analyzed in the current study are available from the corresponding author on reasonable request.
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Acknowledgments
The authors would like to thank Jeonbuk National University for providing clinical samples.
Funding
This work was supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) through the Animal Disease Management Technology Advancement Support Program, funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA) (122012-2, 122060-2) and the research fund of Chungnam National University.
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JHC and JEP designed the experiments. JHB, YML, NDV, and MHK carried out the experiments. JHB, YML, NDV, MHK, JHC, and JEP analyzed the data. JEP wrote the manuscript. JHC, VPL, and JZ edited the manuscript. All of the authors read and approved the final manuscript.
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Baek, J.H., Lee, YM., Vu, N.D. et al. A multiplex real-time RT-qPCR assay for simultaneous detection of porcine epidemic diarrhea virus, porcine deltacoronavirus, and swine acute diarrhea syndrome coronavirus. Arch Virol 169, 82 (2024). https://doi.org/10.1007/s00705-024-06003-9
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DOI: https://doi.org/10.1007/s00705-024-06003-9