A lipidomic and metabolomic signature of a very low-carbohydrate high-fat diet and high-intensity interval training: an additional analysis of a randomized controlled clinical trial
-
Published:2023-12-23
Issue:1
Volume:20
Page:
-
ISSN:1573-3890
-
Container-title:Metabolomics
-
language:en
-
Short-container-title:Metabolomics
Author:
Cipryan LukasORCID, Kosek VitORCID, García Carlos J.ORCID, Dostal TomasORCID, Bechynska KamilaORCID, Hajslova JanaORCID, Hofmann PeterORCID
Abstract
Abstract
Introduction
Regular physical activity and dietary variety are modifiable and influential factors of health outcomes. However, the cumulative effects of these behaviors are not well understood. Metabolomics may have a promising research potential to extend our knowledge and use it in the attempts to find a long-term and sustainable personalized approach in exercise and diet recommendations.
Objective
The main aim was to investigate the effect of the 12 week very low carbohydrate high fat (VLCHF) diet and high-intensity interval training (HIIT) on lipidomic and metabolomic profiles in individuals with overweight and obesity.
Methods
The participants (N = 91) were randomly allocated to HIIT (N = 22), VLCHF (N = 25), VLCHF + HIIT (N = 25) or control (N = 19) groups for 12 weeks. Fasting plasma samples were collected before the intervention and after 4, 8 and 12 weeks. The samples were then subjected to untargeted lipidomic and metabolomic analyses using reversed phase ultra-high-performance liquid chromatography coupled to high-resolution mass spectrometry.
Results
The VLCHF diet affected plasma lipids considerably while the effect of HIIT was unremarkable. Already after 4 weeks of intervention substantial changes of plasma lipids were found in both VLCHF diet groups. The changes persisted throughout the entire 12 weeks of the VLCHF diet. Specifically, acyl carnitines, plasmalogens, fatty acyl esters of hydroxy fatty acid, sphingomyelin, ceramides, cholesterol esters, fatty acids and 4-hydroxybutyric were identified as lipid families that increased in the VLCHF diet groups whereas lipid families of triglycerides and glycerophospholipids decreased. Additionally, metabolomic analysis showed a decrease of theobromine.
Conclusions
This study deciphers the specific responses to a VLCHF diet, HIIT and their combination by analysing untargeted lipidomic and metabolomic profile. VLCHF diet caused divergent changes of plasma lipids and other metabolites when compared to the exercise and control group which may contribute to a better understanding of metabolic changes and the appraisal of VLCHF diet benefits and harms.
Clinical Trial Registry number
NCT03934476, registered 1st May 2019 https://clinicaltrials.gov/ct2/show/NCT03934476?term=NCT03934476&draw=2&rank=1.
Funder
Grantová Agentura České Republiky Ministerstvo Školství, Mládeže a Tělovýchovy University of Chemistry and Technology, Prague
Publisher
Springer Science and Business Media LLC
Subject
Clinical Biochemistry,Biochemistry,Endocrinology, Diabetes and Metabolism
Reference87 articles.
1. Agostoni, C., Boccia, S., Banni, S., Mannucci, P. M., & Astrup, A. (2021). Sustainable and personalized nutrition: From earth health to public health. European Journal of Internal Medicine, 86, 12–16. https://doi.org/10.1016/j.ejim.2021.02.012 2. Akhiyat, N., Vasile, V., Ahmad, A., Sara, J. D., Nardi, V., Lerman, L. O., Allan, J., & Amir, L. (2022). Plasma ceramide levels are elevated in patients with early coronary atherosclerosis and endothelial dysfunction. Journal of the American Heart Association. https://doi.org/10.1161/JAHA.121.022852 3. Alshehry, Z. H., Mundra, P. A., Barlow, C. K., Mellett, N. A., Wong, G., McConville, M. J., Simes, J., Tonkin, A. M., Sullivan, D. R., Barnes, E. H., Nestel, P. J., Kingwell, B. A., Marre, M., Neal, B., Poulter, N. R., Rodgers, A., Williams, B., Zoungas, S., & Meikle, P. J. (2016). Plasma lipidomic profiles improve on traditional risk factors for the prediction of cardiovascular events in type 2 diabetes mellitus. Circulation, 134(21), 1637–1650. https://doi.org/10.1161/CIRCULATIONAHA.116.023233 4. Baci, D., Bruno, A., Cascini, C., Gallazzi, M., Mortara, L., Sessa, F., Pelosi, G., Albini, A., & Noonan, D. M. (2019). Acetyl-L-carnitine downregulates invasion (CXCR4/CXCL12, MMP-9) and angiogenesis (VEGF, CXCL8) pathways in prostate cancer cells: Rationale for prevention and interception strategies. Journal of Experimental & Clinical Cancer Research, 38(1), 464. https://doi.org/10.1186/s13046-019-1461-z 5. Bene, J., Hadzsiev, K., & Melegh, B. (2018). Role of carnitine and its derivatives in the development and management of type 2 diabetes. Nutrition & Diabetes, 8(1), 8. https://doi.org/10.1038/s41387-018-0017-1
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
|
|