Abstract
Abstract
Introduction
Vitiligo pathogenesis is complicated, and several possibilities were suggested. However, it is well-known that the metabolism of pigments plays a significant role in the pathogenicity of the disease.
Objectives
We explored the role of amino acids in vitiligo using targeted metabolomics.
Methods
The amino acid profile was studied in plasma using liquid chromatography. First, 22 amino acids were derivatized and precisely determined. Next, the concentrations of the amino acids and the molar ratios were calculated in 31 patients and 34 healthy individuals.
Results
The differential concentrations of amino acids were analyzed and eight amino acids, i.e., cysteine, arginine, lysine, ornithine, proline, glutamic acid, histidine, and glycine were observed differentially. The ratios of cysteine, glutamic acid, and proline increased significantly in Vitiligo patients, whereas arginine, lysine, ornithine, glycine, and histidine decreased significantly compared to healthy individuals. Considering the percentage of skin area, we also showed that glutamic acid significantly has a higher amount in patients with less than 25% involvement compared to others. Finally, cysteine and lysine are considered promising candidates for diagnosing and developing the disorder with high accuracy (0.96).
Conclusion
The findings are consistent with the previously illustrated mechanism of Vitiligo, such as production deficiency in melanin and an increase in immune activity and oxidative stress. Furthermore, new evidence was provided by using amino acids profile toward the pathogenicity of the disorder.
Funder
Suomalainen Tiedeakatemia
University of Helsinki including Helsinki University Central Hospital
Publisher
Springer Science and Business Media LLC
Subject
Clinical Biochemistry,Biochemistry,Endocrinology, Diabetes and Metabolism
Reference54 articles.
1. Abumrad, N., A. Barbul (2004). The use of arginine in clinical practice. Metabolic and therapeutic aspects of amino acids in clinical nutrition, 595–611
2. Agarwal, S., Mendiratta, V., Chander, R., Jain, A., & Yadav, P. (2015). Study of serum levels of Vitamin B12, folic acid, and homocysteine in vitiligo. Pigment International, 2, 76.
3. Akoglu, G., Neselioglu, S., Karaismailoglu, E., Aktas, A., & Erel, O. (2018). Plasma thiol levels are associated with disease severity in nonsegmental vitiligo. Indian Journal of Dermatology, 63, 323.
4. Amiel, J. M., & Mathew, S. J. (2007). Glutamate and anxiety disorders. Current Psychiatry Reports, 9, 278–283. https://doi.org/10.1007/s11920-007-0033-7
5. Armstrong, A. (2011). Advances in Malignant Melanoma: Clinical and Research Perspectives, BoD–Books on Demand.
Cited by
7 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献