Gum Arabic influences the activity of antioxidant enzymes during androgenesis in barley anthers

Author:

Klajmon A.,Makowska K.,Zimny J.,Oleszczuk S.,Libik-Konieczny M.,Sebela M.,Gašparíková I.,Bąba W.,Konieczny R.ORCID

Abstract

AbstractThis study investigated the antioxidant activity of gum Arabic (GA) during androgenesis in barley anthers. After stress pretreatment the anthers were cultured in the presence of 10 mg L−1 GA (Gm) or on control medium (Cm) and compared with respect to activity of selected antioxidant and respiratory enzymes and endogenous hydrogen peroxide (H2O2) content. The anthers from Cm and Gm differed in the strategy against H2O2 overproduction, in that the total peroxidase (POX) activity and the number of POX isoforms were significantly higher in Gm- than in Cm-cultured material. High POX activity on Gm paralleled with H2O2 decrease, suggesting the utilization of this chemical for the POX-mediated cell wall formation and reconstruction during growth of multicellular structures. The total superoxide dismutase (SOD) activity on Cm and Gm were at similar level for most of the culture period but the activity of MnSOD was dozen times higher on Gm and this coincided with high activity of fumarase and cytochrome c oxidase. It indicates close interplay between efficient antioxidative protection and high metabolic rate accompanying efficient androgenesis. Mass spectrometry analysis confirmed the presence of POX and other antioxidative and defense enzymes in protein fraction of GA used in the experiments, however as revealed by 2,2-diphenyl-1-picrylhydrazyl assay, the Gm and Cm displayed similar total antioxidant capacity. Thus, the effect of GA on androgenic cultures of barley can be linked to its influence on the activity of anther antioxidant system, rather than its native antioxidative properties.

Publisher

Springer Science and Business Media LLC

Subject

Horticulture

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3