Integrated omics analysis reveals the alteration of gut microbiota and fecal metabolites in Cervus elaphus kansuensis
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Published:2024-01-15
Issue:1
Volume:108
Page:
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ISSN:0175-7598
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Container-title:Applied Microbiology and Biotechnology
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language:en
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Short-container-title:Appl Microbiol Biotechnol
Author:
Zhang ZhenxiangORCID, Bao ChanghongORCID, Li ZhaonanORCID, He CaixiaORCID, Jin WenjieORCID, Li ChangzhongORCID, Chen YanxiaORCID
Abstract
Abstract
The gut microbiota is the largest and most complex microecosystem in animals. It is influenced by the host’s dietary habits and living environment, and its composition and diversity play irreplaceable roles in animal nutrient metabolism, immunity, and adaptation to the environment. Although the gut microbiota of red deer has been studied, the composition and function of the gut microbiota in Gansu red deer (Cervus elaphus kansuensis), an endemic subspecies of red deer in China, has not been reported. In this study, the composition and diversity of the gut microbiome and fecal metabolomics of C. elaphus kansuensis were identified and compared for the first time by using 16S rDNA sequencing, metagenomic sequencing, and LC-MS/MS. There were significant differences in gut microbiota structure and diversity between wild and farmed C. elaphus kansuensis. The 16S rDNA sequencing results showed that the genus UCRD-005 was dominant in both captive red deer (CRD) and wild red deer (WRD). Metagenomic sequencing showed similar results to those of 16S rDNA sequencing for gut microbiota in CRD and WRD at the phylum and genus levels. 16S rDNA and metagenomics sequencing data suggested that Bacteroides and Bacillus might serve as marker genera for CRD and WRD, respectively. Fecal metabolomics results showed that 520 metabolites with significant differences were detected between CRD and WRD and most differential metabolites were involved in lipid metabolism. The results suggested that large differences in gut microbiota composition and fecal metabolites between CRD and WRD, indicating that different dietary habits and living environments over time have led to the development of stable gut microbiome characteristics for CRD and WRD to meet their respective survival and reproduction needs.
Key points
• Environment and food affected the gut microbiota and fecal metabolites in red deer
• Genera Bacteroides and Bacillus may play important roles in CRD and WRD, respectively
• Flavonoids and ascorbic acid in fecal metabolites may influence health of red deer
Funder
Science and Technology Innovation Project of Three RiverSource First-class Discipline, College of Eco–Environment Engineering, Qinghai University Doctoral Scientific Research Start-up Foundation of Qinghai University
Publisher
Springer Science and Business Media LLC
Subject
Applied Microbiology and Biotechnology,General Medicine,Biotechnology
Reference110 articles.
1. (FEEDAP) EPoAaPoSuiAF, Bampidis V, Azimonti G, Bastos ML, Christensen H, Dusemund B, Fasmon Durjava M, Kouba M, Lopez-Alonso M, Lopez Puente S, Marcon F, Mayo B, Pechova A, Petkova M, Ramos F, Sanz Y, Villa RE, Woutersen R, Anguita M et al (2022) Safety and efficacy of a feed additive consisting of riboflavin-5’-phosphate ester monosodium salt (vitamin B(2)) (from riboflavin 98%, produced by Bacillus subtilis KCCM 10445) for all animal species (Hubei Guangji Pharmaceutical Co. Ltd). EFSA J 20(11):e07608. https://doi.org/10.2903/j.efsa.2022.7608 2. Adeniji AA, Loots DT, Babalola OO (2019) Bacillus velezensis: phylogeny, useful applications, and avenues for exploitation. Appl Microbiol Biotechnol 103(9):3669–3682. https://doi.org/10.1007/s00253-019-09710-5 3. Akula R, Mukherjee S (2020) New insights on neurotransmitters signaling mechanisms in plants. Plant Signal Behav 15(6):1737450. https://doi.org/10.1080/15592324.2020.1737450 4. Antwis RE, Edwards KL, Unwin B, Walker SL, Shultz S (2019) Rare gut microbiota associated with breeding success, hormone metabolites and ovarian cycle phase in the critically endangered eastern black rhino. Microbiome 7(1):27. https://doi.org/10.1186/s40168-019-0639-0 5. Arumugam M, Raes J, Pelletier E, Le Paslier D, Yamada T, Mende DR, Fernandes GR, Tap J, Bruls T, Batto JM, Bertalan M, Borruel N, Casellas F, Fernandez L, Gautier L, Hansen T, Hattori M, Hayashi T, Kleerebezem M et al (2011) Enterotypes of the human gut microbiome. Nature 473(7346):174–180. https://doi.org/10.1038/nature09944
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