Exosomes Derived from Adipose Stem Cells Enhance Angiogenesis in Diabetic Wound Via miR-146a-5p/JAZF1 Axis-Reference-Cited by-同舟云学术

Exosomes Derived from Adipose Stem Cells Enhance Angiogenesis in Diabetic Wound Via miR-146a-5p/JAZF1 Axis

Published:2024-02-23 Issue:4 Volume:20 Page:1026-1039
ISSN:2629-3269
Container-title:Stem Cell Reviews and Reports
language:en
Short-container-title:Stem Cell Rev and Rep

Author:

Che Dehui,Xiang Xinjian,Xie Juan,Chen Zenghong,Bao Qiong,Cao Dongsheng^ORCID

Abstract

AbstractChronic trauma in diabetes is a leading cause of disability and mortality. Exosomes show promise in tissue regeneration. This study investigates the role of exosomes derived from adipose stem cells (ADSC-Exos) in angiogenesis. MiRNA-seq analysis revealed significant changes in 47 genes in human umbilical vein endothelial cells (HUVECs) treated with ADSC-Exos, with miR-146a-5p highly expressed. MiR-146a-5p mimics enhanced the pro-angiogenic effects of ADSC-Exos, while inhibitors had the opposite effect. JAZF1 was identified as a direct downstream target of miR-146a-5p through bioinformatics, qRT-PCR, and dual luciferase assay. Overexpress of JAZF1 resulted in decreased proliferation, migration, and angiogenic capacity of HUVECs, and reduced VEGFA expression. This study proposes that ADSC-Exos regulate angiogenesis partly via the miR-146a-5p/JAZF1 axis. Graphical Abstract

Publisher

Springer Science and Business Media LLC

Link

https://link.springer.com/content/pdf/10.1007/s12015-024-10685-8.pdf

Reference38 articles.

1. Rani, S., & Ritter, T. (2016). The Exosome - A Naturally Secreted Nanoparticle and its Application to Wound Healing. Advanced Materials, 28(27), 5542–5552. https://doi.org/10.1002/adma.201504009

2. Zhao, J., Yang, S., Shu, B., Chen, L., Yang, R., Xu, Y., et al. (2021). Transient high glucose causes persistent vascular dysfunction and delayed wound healing by the DNMT1-mediated ang-1/NF-kappaB pathway. Journal of Investigative Dermatology, 141(6), 1573–1584. https://doi.org/10.1016/j.jid.2020.10.023

3. Yu, M., Huang, J., Zhu, T., Lu, J., Liu, J., Li, X., et al. (2020). Liraglutide-loaded PLGA/gelatin electrospun nanofibrous mats promote angiogenesis to accelerate diabetic wound healing via the modulation of miR-29b-3p. Biomaterials Science, 8(15), 4225–4238. https://doi.org/10.1039/d0bm00442a

4. Kant, V., Gopal, A., Kumar, D., Pathak, N. N., Ram, M., Jangir, B. L., et al. (2015). Curcumin-induced angiogenesis hastens wound healing in diabetic rats. Journal of Surgical Research, 193(2), 978–988. https://doi.org/10.1016/j.jss.2014.10.019

5. Casado-Diaz, A., Quesada-Gomez, J. M., & Dorado, G. (2020). Extracellular vesicles derived from mesenchymal stem cells (MSC) in regenerative medicine: Applications in skin wound healing. Frontiers in Bioengineering and Biotechnology, 8, 146. https://doi.org/10.3389/fbioe.2020.00146

Cited by 1 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Adipose-Derived Stem Cell Exosomes Facilitate Diabetic Wound Healing: Mechanisms and Potential Applications;International Journal of Nanomedicine;2024-06