Author:
Bao Chenchen,Chen Lei,Wang Tao,Lei Chong,Tian Furong,Cui Daxiang,Zhou Yong
Abstract
Abstract
RGD peptides has been used to detect cell surface integrin and direct clinical effective therapeutic drug selection. Herein we report that a quick one step detection of cell surface marker that was realized by a specially designed NiFe-based magnetic biosensing cell chip combined with functionalized magnetic nanoparticles. Magnetic nanoparticles with 20-30 nm in diameter were prepared by coprecipitation and modified with RGD-4C, and the resultant RGD-functionalized magnetic nanoparticles were used for targeting cancer cells cultured on the NiFe-based magnetic biosensing chip and distinguish the amount of cell surface receptor-integrin. Cell lines such as Calu3, Hela, A549, CaFbr, HEK293 and HUVEC exhibiting different integrin expression were chosen as test samples. Calu3, Hela, HEK293 and HUVEC cells were successfully identified. This approach has advantages in the qualitative screening test. Compared with traditional method, it is fast, sensitive, low cost, easy-operative, and needs very little human intervention. The novel method has great potential in applications such as fast clinical cell surface marker detection, and diagnosis of early cancer, and can be easily extended to other biomedical applications based on molecular recognition.
Publisher
Springer Science and Business Media LLC
Subject
Electrical and Electronic Engineering,Surfaces, Coatings and Films,Electronic, Optical and Magnetic Materials
Reference33 articles.
1. C. Gui, X. Dai and D. Cui, “Advances of nanotech- nology applied to biosensors”, Nano Biomed. Eng. 3(4), 260–273 (2011). http://dx.doi.org/10.5101/nbe.v3i4.p260-273
2. J. Chen, D. Chen, T. Yuan and X. Chen, “Microfluidic PCR Chips”, Nano Biomed. Eng. 3 (4), 203–210 (2011). http://dx.doi.org/10.5101/nbe.v3i4.p203-210
3. R. Vijayakumar, S. Jagannathan, P. R. Gandhi and S. Chaansha, “Nanorobotics: a newer platform for molecular diagnose”, Nano Biomed. Eng. 3(3), 192–201 (2011). http://dx.doi.org/10.5101/nbe.v3i3.p192-201
4. X. Zhang, L. Jiang, C. Zhang, D. Li, C. Wang, F. Gao and D. Cui, “A silicon dioxide modified magnetic nanoparticles labeled lateral flow strips for HBs antigen”, J. Biomed. Nanotechnol. 7(6), 776–781 (2011). http://dx.doi.org/10.1166/jbn.2011.1352
5. R. Bashir, “BioMEMS: state-of-the-art in detection, opportunities and prospects”, Adv. Drug Deliv. Rev. 56(11), 1565–1586 (2004). http://dx.doi.org/10.1016/j.addr.2004.03.002.
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