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Determination of Copper Amine Oxidase Activity in Plant Tissues

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Polyamines

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1694))

Abstract

Copper amine oxidases (CuAOs) involved in polyamine catabolism are emerging as physiologically relevant enzymes for their involvement in plant growth, differentiation and defence responses to biotic and abiotic stress. In this chapter, we describe two spectrophotometric and one polarographic method for determining CuAO activity in plant tissues. Some aspects related to cell wall association of apoplastic CuAOs and possible interference of plant metabolites with the enzymatic activity assays are also considered.

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Authors and Affiliations

  1. Department of Science, University “Roma Tre”, Viale Guglielmo Marconi 446, 00146, Rome, Italy

    Riccardo Angelini, Alessandra Cona & Paraskevi Tavladoraki

Authors
  1. Riccardo Angelini
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  2. Alessandra Cona
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  3. Paraskevi Tavladoraki
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Corresponding author

Correspondence to Riccardo Angelini .

Editor information

Editors and Affiliations

  1. Department of Biology, Healthcare and Environment, Faculty of Pharmacy and Food Sciences, Section of Plant Physiology, University of Barcelona, Spain

    Rubén Alcázar

  2. Department of Biology, Healthcare and Environment, Faculty of Pharmacy and Food Sciences, Section of Plant Physiology, University of Barcelona, Spain

    Antonio F. Tiburcio

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Angelini, R., Cona, A., Tavladoraki, P. (2018). Determination of Copper Amine Oxidase Activity in Plant Tissues. In: Alcázar, R., Tiburcio, A. (eds) Polyamines. Methods in Molecular Biology, vol 1694. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7398-9_13

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  • DOI: https://doi.org/10.1007/978-1-4939-7398-9_13

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7397-2

  • Online ISBN: 978-1-4939-7398-9

  • eBook Packages: Springer Protocols

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