Abstract
Abstract
Background
Membranous nephropathy (MN) and IgA nephropathy (IgAN) are the most common primary glomerulopathies worldwide. The systemic metabolic changes in the progression of MN and IgAN are not fully understood.
Methods
A total of 87 and 70 patients with MN and IgAN, respectively, and 30 healthy controls were enrolled in this study. Untargeted metabolomics was performed to explore the differential metabolites and metabolic pathways in the early stage of MN and IgAN. To judge the diagnostic ability of biomarkers, receiver operating characteristic curve analysis (ROC) were performed.
Results
Principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA) suggested that patients with MN and IgAN showed an obvious separation trend from the healthy controls. In addition, 155 and 148 metabolites were identified to be significantly altered in the MN and IgAN groups, respectively. Of these, 70 metabolites were markedly altered in both disease groups; six metabolites, including L-tryptophan, L-kynurenine, gamma-aminobutyric acid (GABA), indoleacetaldehyde, 5-hydroxyindoleacetylglycine, and N-alpha-acetyllysine, showed the opposite tendency. The most affected metabolic pathways included the amino acid metabolic pathways, citrate cycle, pantothenate and CoA biosynthesis, and hormone signaling pathways.
Conclusions
Substantial metabolic disorders occurred during the progression of MN and IgAN. L-tryptophan, L-kynurenine, GABA, indoleacetaldehyde, 5-hydroxyindoleacetylglycine, and N-alpha-acetyllysine may show potential as biomarkers for the identification of MN and IgAN.
Funder
Suzhou Pharmaceutical Association
Publisher
Springer Science and Business Media LLC
Reference25 articles.
1. Zhang L, Wang F, Wang L, Wang W, Liu B, Liu J et al (2012) Prevalence of chronic kidney disease in China: a cross-sectional survey. Lancet 379:815–822
2. Pippias M, Kramer A, Noordzij M, Afentakis N, de la Torre RA, Ambühl PM et al (2017) The European renal association—European dialysis and transplant association registry annual report 2014: a summary. Clin Kidney J 10:154–169
3. Krata N, Foroncewicz B, Zagożdżon R, Moszczuk B, Zielenkiewicz M, Pączek L et al (2021) Peroxiredoxins as markers of oxidative stress in IgA nephropathy, membranous nephropathy and lupus nephritis. Arch Immunol Ther Exp (Warsz) 70:3
4. Fiehn O (2002) Metabolomics–the link between genotypes and phenotypes. Plant Mol Biol 48:155–171
5. Rinschen MM, Ivanisevic J, Giera M, Siuzdak G (2019) Identification of bioactive metabolites using activity metabolomics. Nat Rev Mol Cell Biol 20:353–367
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