Author:
Keresztény Tibor,Libisch Balázs,Orbe Stephanya Corral,Nagy Tibor,Kerényi Zoltán,Kocsis Róbert,Posta Katalin,Papp Péter P.,Olasz Ferenc
Abstract
AbstractLactic acid bacteria (LAB) in the microbiota play an important role in human and animal health and, when used as probiotics, can contribute to an increased growth performance in livestock management. Animals living in their native habitat can serve as natural sources of microorganisms, so isolation of LAB strains from wild boars could provide the opportunity to develop effective probiotics to improve production in swine industry. In this study, the probiotic potential of 56 LAB isolates, originated from the ileum, colon, caecum and faeces of 5 wild boars, were assessed in vitro in details. Their taxonomic identity at species level and their antibacterial activity against four representative strains of potentially pathogenic bacteria were determined. The ability to tolerate low pH and bile salt, antibiotic susceptibility, bile salt hydrolase activity and lack of hemolysis were tested. Draft genome sequences of ten Limosilactobacillus mucosae and three Leuconostoc suionicum strains were determined. Bioinformatic analysis excluded the presence of any known acquired antibiotic resistance genes. Three genes, encoding mesentericin B105 and two different bacteriocin-IIc class proteins, as well as two genes with possible involvement in mesentericin secretion (mesE) and transport (mesD) were identified in two L. suionicum strains. Lam29 protein, a component of an ABC transporter with proved function as mucin- and epithelial cell-adhesion factor, and a bile salt hydrolase gene were found in all ten L. mucosae genomes. Comprehensive reconsideration of all data helps to select candidate strains to assess their probiotic potential further in animal experiments.
Funder
Hungarian National Research, Development and Innovation Office
European Commission through the NeoGiant Horizon 2020
Hungarian National Laboratory Project
Hungarian University of Agriculture and Life Sciences
Publisher
Springer Science and Business Media LLC
Subject
Molecular Biology,Molecular Medicine,Microbiology
Reference103 articles.
1. European Parliament and the Council of the European Union (2008) Commission Regulation (EC) No 429/2008 of 25 April 2008 on detailed rules for the implementation of Regulation (EC) No 1831/2003 of the European Parliament and of the Council as regards the preparation and the presentation of applications and the assessment and the authorisation of feed additives Off J Eur Union L133:1-653. http://data.europa.eu/eli/reg/2008/429/oj
2. Dowarah R, Verma AK, Agarwal N (2017) The use of Lactobacillus as an alternative of antibiotic growth promoters in pigs: A review. Anim Nutr 3:1–6. https://doi.org/10.1016/j.aninu.2016.11.002
3. Sirichokchatchawan W, Pupa P, Praechansri P, Am-in N, Tanasupawat S, Sonthayanon P, Prapasarakula N (2018) Autochthonous lactic acid bacteria isolated from pig faeces in Thailand show probiotic properties and antibacterial activity against enteric pathogenic bacteria. Microbial Pathogenesis 119:208–215. https://doi.org/10.1016/j.micpath.2018.04.031
4. Devi SM, Archer AC, Halami PM (2015) Screening, characterization and in vitro evaluation of probiotic properties among lactic acid bacteria through comparative analysis. Probiotics Antimicro Prot 7:181–192. https://doi.org/10.1007/s12602-015-9195-5
5. Zhang Z, Lv J, Pan L, Zhang Y (2018) Roles and applications of probiotic Lactobacillus strains. Appl Microbiol Biotechnol 102:8135–8143. https://doi.org/10.1007/s00253-018-9217-9
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献