Molecular detection of viruses in Kenyan bats and discovery of
novel astroviruses, caliciviruses and rotaviruses
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Published:2017-04
Issue:2
Volume:32
Page:101-114
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ISSN:1674-0769
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Container-title:Virologica Sinica
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language:en
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Short-container-title:Virol. Sin.
Author:
Waruhiu Cecilia,Ommeh Sheila,Obanda Vincent,Agwanda Bernard,Gakuya Francis,Ge Xing-Yi,Yang Xing-Lou,Wu Li-Jun,Zohaib Ali,Hu Ben,Shi Zheng-Li
Abstract
AbstractThis is the first country-wide surveillance of bat-borne viruses in Kenya spanning
from 2012–2015 covering sites perceived to have medium to high level bat-human
interaction. The objective of this surveillance study was to apply a non-invasive
approach using fresh feces to detect viruses circulating within the diverse species
of Kenyan bats. We screened for both DNA and RNA viruses; specifically, astroviruses
(AstVs), adenoviruses (ADVs), caliciviruses (CalVs), coronaviruses (CoVs),
flaviviruses, filoviruses, paramyxoviruses (PMVs), polyomaviruses (PYVs) and
rotaviruses. We used family-specific primers, amplicon sequencing and further
characterization by phylogenetic analysis. Except for filoviruses, eight virus
families were detected with varying distributions and positive rates across the five
regions (former provinces) studied. AstVs (12.83%), CoVs (3.97%), PMV (2.4%), ADV
(2.26%), PYV (1.65%), CalVs (0.29%), rotavirus (0.19%) and flavivirus (0.19%). Novel
CalVs were detected in Rousettus aegyptiacus and
Mops condylurus while novel
Rotavirus-A-related viruses were detected in Taphozous bats and R.
aegyptiacus. The two Rotavirus A (RVA)
strains detected were highly related to human strains with VP6 genotypes I2 and I16.
Genotype I16 has previously been assigned to human RVA-strain B10 from Kenya only,
which raises public health concern, particularly considering increased human-bat
interaction. Additionally, 229E-like bat CoVs were detected in samples originating
from Hipposideros bats roosting in sites with
high human activity. Our findings confirm the presence of diverse viruses in Kenyan
bats while providing extended knowledge on bat virus distribution. The detection of
viruses highly related to human strains and hence of public health concern,
underscores the importance of continuous surveillance.
Subject
Virology,Molecular Medicine,Immunology
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