Previous stress causes a contrasting response to cadmium toxicity in the aquatic snail Potamopyrgus antipodarum: lethal and behavioral endpoints

Abstract

Abstract In aquatic ecosystems, animals are often exposed to a combination of stressors, including both natural and anthropogenic factors. Combined stressors may have additive or interactive effects on animals, either magnifying or reducing the effects caused by each stressor alone. Therefore, standardized bioassays can lead to overestimations or underestimations of the risk of toxicants if natural stressors are not bear in mind. The inclusion of natural stress in laboratory bioassays may help to extrapolate the laboratory results to ecosystems. This study assesses the effects of successive exposure to two sources of stress (high water conductivity and cadmium toxicity) on the behavior and survival of the aquatic snail Potamopyrgus antipodarum (Tateidae, Mollusca). I conducted a bioassay consisting on exposure to high conductivity (5000 mg NaCl/L, 7 days), followed by exposure to cadmium (0.03, 0.125, and 0.25 mg Cd/L for 7 days) and by a post-exposure period (7 days). Mortality, inactivity, and the time to start activity of active animals were monitored in each animal. In general, cadmium lethality was higher in animals previously undergoing high conductivity than in non-stressed ones. Previously stressed animals showed longer time to start activity, with a noticeable effect at the two highest cadmium concentrations. Animals submitted to the two highest cadmium concentration both, stressed and non-stressed, showed a moderate recovery during the post-exposure period. It is concluded that previous stress caused a worsening of the cadmium toxicity on the aquatic snail Potamopyrgus antipodarum, which is especially noticeable for mortality. However, there was no interactive effect between cadmium and conductivity on snail activity, which may be indicative of recovery after cadmium exposure regardless the previous stress suffered by the snails.