Abstract
The interest in algae-derived bioactive compounds has grown due to their potential therapeutic efficacy against a range of diseases. These compounds, derived from proteins, exhibit diverse functions and profound pharmacological effects. Recent research has highlighted the extensive health benefits of algae-derived bioactive compounds, positioning them as potential natural antioxidants in the food, pharmaceutical, and cosmetic industries. This study focuses on extracting proteins from Porphyra yezoensis using innovative physical pre-treatment methods such as stirring, ball milling, and homogenization, under various acidic and alkaline conditions. Enzymatic hydrolysis, employing commercial enzymes at optimal temperature, pH, and enzyme–substrate ratios, produced distinct fractions according to molecular weight. Pepsin demonstrated the highest hydrolysis rate, with the fraction above 10 kDa identified as the most bioactive hydrolysate. Antioxidant activity was evaluated through DPPH, ABTS, ferrous ion chelation, and reducing power assays, demonstrating high antioxidant potential and the ability to mitigate oxidative stress. The 10 kDa fraction of pepsin hydrolysate exhibited 82.6% DPPH activity, 77.5% ABTS activity, 88.4% ferrous ion chelation activity, and higher reducing power potential (0.84 absorbance at 700 nm). Further exploration of mechanisms, amino acid profiles, and potential in vivo benefits is essential to fully exploit the medicinal potential of these algae-derived hydrolysates.
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Abbreviations
- ABTS:
-
2,2′-Azino-bis -3-ethylbenzothiazoline-6-sulfonic acid
- BHA:
-
Butylated hydroxyanisole
- DPPH:
-
2,2-Diphenyl-1-picrylhdrazyl
- TPC:
-
Total Phenol Content
- kDa:
-
Kilo Dalton
- DH:
-
Degree of hydrolysis
- TNBS:
-
2,4,6-Trinitrobenzene sulfonic acid
- EDTA:
-
Ethylenediaminetetraacetic acid
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Acknowledgements
The authors acknowledge National Science and Technology Council, Taiwan for this study
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AKP is grateful to NSTC, Taiwan for funding support (Ref. No. NSTC 111–2222-E-992–006).
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HMW: Writing—original draft, literature review; C-YH: Supervision, draft preparation, lab space; C-WC: Supervision, draft preparation; BSG, RRS: Supervision, Writing—review, and editing.; AKP: Supervision, funding, Writing—review and editing; C-DD: Supervision, lab space, Writing—review and editing.
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Wani, H.M., Huang, CY., Singhania, R.R. et al. Assessing and optimizing the bioactivities of diverse enzyme-derived protein hydrolysates from Porphyra yezoensis: unlocking the health potential. J Food Sci Technol (2024). https://doi.org/10.1007/s13197-024-05935-z
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DOI: https://doi.org/10.1007/s13197-024-05935-z