Affiliation:
1. This investigation is part of the medical research project Diabetes Mellitus and Disturbances of Lipid Metabolism Ministry of Health, GDR
Abstract
To investigate changes in the sensitivity of early insulin repsonse to glucose, a pulse stimulation of 1.0, 2.5, and 5.0 g glucose was carried out at intervals of 30 min in 73 nonobese subjects suspected of having an impaired carbohydrate tolerance (CHT) and 24 body-weight and sex-matched healthy controls without a family history of diabetes. CHT was characterized by means of a 2-h glucose infusion test (GIT; 12 mg/kg-min), primed by an initial injection of 0.33 g/kg. Subjects suspected of having an impaired CHT (glucosuria in all subjects, 49 of whom exibited a borderline or pathologic 50-g standard oral glucose tolerance test and 27 of whom had a family history of diabetes) were classified as having a normal, borderline, and pathologic CHT according to the result of GIT. Compared with controls the early insulin response (∆IRI area, 0–5 min) during GIT was significantly reduced in subjects with normal, borderline, and pathologic CHT. During staircase glucose stimulation, a dose-dependent significant increase of the ∆max IRI response was observed in controls, whereas this strong relationship was lacking in all other groups studied. In subjects with normal, borderline, and pathologic CHT, ∆max IRI responses revealed by pulse stimulation of 1.0, 2.5, and 5.0 g glucose were significantly reduced compared with controls. Besides quantitative differences in early insulin response among all groups studied, a shift of the glucoseinsulin dose-response curve to the right was found in subjects with normal, borderline, and pathologic CHT. Our results provide further evidence of a reduced sensitivity of the pancreatic beta-cells to glucose during development of carbohydrate intolerance. The staircase glucose stimulation enables both a quantitative evaluation of the early insulin response to glucose and a characterization of the glucose-insulin dose-response relationship. For this reason the test seems to be a useful tool in studying the sensitivity of the pancreatic beta-cells to glucose.
Publisher
American Diabetes Association
Subject
Advanced and Specialized Nursing,Endocrinology, Diabetes and Metabolism,Internal Medicine
Cited by
27 articles.
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