Affiliation:
1. Medical Clinic and Policlinic, University of Giessen Germany
Abstract
Objective
In this study, ingestion of Staphylococcus aureus and “bacteria killing” (BK) were measured to evaluate polymorphonuclear leukocyte (PMN) phagocytic functions and chemiluminescence response (CL) to phorbolmyristic acetate (PMA) as respiratory burst activity with regard to metabolic control parameters in diabetic patients.
Research Design and Methods
PMN phagocytic functions were assessed in 40 diabetic patients, all receiving insulin and in poor metabolic control, with 3H-thymidine-labeled Staphylococcus aureus in a modified radiometric assay. Bacteria killing was determined by pure-plate counting of surviving bacteria (colony-forming units [cm]) and luminol-enhanced CL in response to PMA as a measure of respiratory burst. PMN function data were correlated to HbA1 as parameter of recent metabolic control.
Results
PMN of diabetic patients showed a significant reduction in Staphylococcus aureus (50.7 ± 4.1%) and BK (29.4 ± 4.2%) compared with healthy nondiabetic control subjects (76.6 ± 4.6% and 16.3 ± 3.1%, respectively, P < 0.001), and PMN CL response was markedly reduced in diabetic patients also. Linear regression analysis showed a highly significant negative correlation of HbAi versus Staphylococcus aureus (r = -0.67, P = 0.001) and a positive correlation for BK (r = 0.73, P < 0.001). This was also true for CL, although this did not reach statistical significance (P = 0.06).
Conclusions
The data obtained demonstrate impaired PMN phagocytic functions and CL response in diabetic patients. These findings suggest inhibitory effects of elevated glucose concentrations on PMNs, a possible role of protein glycosylation for impairing PMN function, thus contributing in part to altered host defense.
Publisher
American Diabetes Association
Subject
Advanced and Specialized Nursing,Endocrinology, Diabetes and Metabolism,Internal Medicine
Cited by
291 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献